The effects of low nitrate levels on the freshwater cyanobacterium Synechocystis sp. strain PCC 6803: Construction of a bioreporter assay and molecular characterization by transcriptome and proteome analysis
نویسنده
چکیده
2003 2 3 PLEDGE I certify that the present thesis entitled: " The effects of low nitrate levels on the freshwater cyanobacterium Synechocystis sp. strain PCC 6803: Construction of a bioreporter assay and molecular characterization by transcriptome and proteome analysis " was carried out without any unlawful devices. I did not use any other than the described literature sources or technical devices. This work has never been submitted before in this or similar form to any other university and has not been used for any examination. ACKNOWLEDGEMENTS I would like to thank all those who contrib uted in one way or another to the research summarized in this thesis, first of all my supervisor, Professor Dr. Rolf D. Schmid for giving me the opportunity to do this work in his laboratory and also for his useful advise and encouragement. Furthermore, I would like to thank him very much for his support to my scholarship application. I would also like to thank Dr. Till T. Bachmann, the head of the Biosensor group at the ITB, for his guidance, enthousiasm and numerous helpful discussions, corrections of m y publications and the manuscript of this dissertation. I am grateful to all my colleagues at ITB and especially the members of the Biosensor group, for every kind of support they brought to me during my work.
منابع مشابه
Expression of the ggpS gene, involved in osmolyte synthesis in the marine cyanobacterium Synechococcus sp. Strain PCC 7002, revealed regulatory differences between this strain and the freshwater strain Synechocystis sp. Strain PCC 6803.
Synthesis of the osmolyte glucosylglycerol (GG) in the marine cyanobacterium Synechococcus sp. strain PCC 7002 was characterized. The ggpS gene, which encodes the key enzyme (GG-phosphate synthase [GgpS]) in GG biosynthesis, was cloned by using PCR. A 2,030-bp DNA sequence which contained one open reading frame (ORF) was obtained. The protein deduced from this ORF exhibited 85% similarity to th...
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Water use assessment is important for bioproduction using cyanobacteria. For eco-friendly reasons, seawater should preferably be used for cyanobacteria cultivation instead of freshwater. In this study, we demonstrated that the freshwater unicellular cyanobacterium Synechocystis sp. PCC 6803 could be grown in a medium based on seawater. The Synechocystis wild-type strain grew well in an artifici...
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Ultrastructural and immunocytochemical investigations gave evidence that cyanophycin (multi-L-arginyl-poly-L-aspartate) granules accumulate in the cyanobacterium Synechocystis sp. strain PCC 6803 under nutrient deficient growth conditions, especially under phosphate limitation. Besides nutrient deficiency, growth of Synechocystis PCC 6803 on L-arginine or L-asparagine as sole N-source also led ...
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The cyanobacterium Synechocystis sp. strain PCC 6803 is able to acclimate to levels of salinity ranging from freshwater to twice the seawater concentrations of salt by accumulating the compatible solute glucosylglycerol (GG). Expression of the ggpS gene coding for the key enzyme (glucosylglycerol-phosphate synthase) in GG synthesis was examined in detail. Under control conditions, the GgpS prot...
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